PDBe 1xiw

X-ray diffraction
1.9Å resolution

Crystal structure of human CD3-e/d dimer in complex with a UCHT1 single-chain antibody fragment

Released:
Source organisms:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
immunoglobulin heavy chain variable region Chains: D, H
Molecule details ›
Chains: D, H
Length: 122 amino acids
Theoretical weight: 13.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
immunoglobulin light chain variable region Chains: C, G
Molecule details ›
Chains: C, G
Length: 108 amino acids
Theoretical weight: 11.99 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
T-cell surface glycoprotein CD3 delta chain Chains: B, F
Molecule details ›
Chains: B, F
Length: 79 amino acids
Theoretical weight: 9.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04234 (Residues: 22-100; Coverage: 53%)
Gene names: CD3D, T3D
Structure domains: Immunoglobulins
T-cell surface glycoprotein CD3 epsilon chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 105 amino acids
Theoretical weight: 11.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07766 (Residues: 22-126; Coverage: 56%)
Gene names: CD3E, T3E
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 64.874Å b: 79.326Å c: 150.747Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.204 0.241
Expression system: Escherichia coli BL21(DE3)