PDBe 1xa9

X-ray diffraction
2.5Å resolution

Crystal structure of yellow fluorescent protein zFP538 K66M green mutant


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
GFP-like fluorescent chromoprotein FP538 Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 26.19 KDa
Source organism: Zoanthus sp.
Expression system: Escherichia coli
  • Canonical: Q9U6Y4 (Residues: 1-231; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P6222
Unit cell:
a: 108.432Å b: 108.432Å c: 84.924Å
α: 90° β: 90° γ: 120°
R R work R free
0.19 0.176 0.295
Expression system: Escherichia coli