PDBe 1x75

X-ray diffraction
2.8Å resolution

CcdB:GyrA14 complex

Released:
Source organism: Escherichia coli

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA gyrase subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 14.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AES4 (Residues: 363-494; Coverage: 15%)
Gene names: JW2225, b2231, gyrA, hisW, nalA, parD
Structure domains: Topoisomerase, domain 3
Toxin CcdB Chains: C, D
Molecule details ›
Chains: C, D
Length: 101 amino acids
Theoretical weight: 11.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P62554 (Residues: 1-101; Coverage: 100%)
Gene names: ECOK12F043, G, ccdB, letB, letD
Sequence domains: CcdB protein
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 52.124Å b: 83.344Å c: 110.919Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.224 0.298
Expression system: Escherichia coli