PDBe 1wsr

X-ray diffraction
2Å resolution

Crystal Structure of Human T-protein of Glycine Cleavage System

Released:

Function and Biology Details

Reaction catalysed:
[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH(3)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminomethyltransferase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 40.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P48728 (Residues: 29-403; Coverage: 93%)
Gene names: AMT, GCST
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 54.17Å b: 128.52Å c: 136.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 0.193
Expression system: Escherichia coli