› 1wqs

X-ray diffraction
2.8Å resolution

Crystal structure of Norovirus 3C-like protease

Released:
DOI: 10.2210/pdb1wqs/pdb
PDB entry 1wqs coloured by chain, front view.
PDB entry 1wqs coloured by chain, side view.
PDB entry 1wqs coloured by chain, top view.
Source organism: Chiba virus
Primary publication:
A norovirus protease structure provides insights into active and substrate binding site integrity.
Nakamura K, Someya Y, Kumasaka T, Ueno G, Yamamoto M, Sato T, Takeda N, Miyamura T, Tanaka N
J Virol 79 13685-93 (2005)
PMID: 16227288

Function and Biology Details

Reaction catalysed: 
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190120 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3C-like protease Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 173 amino acids
Theoretical weight: 18.58 KDa
Source organism: Chiba virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9DU47 (Residues: 1097-1269; Coverage: 10%)
Gene name: ORF1
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
Ligand HG 8 x HG
Ligand TLA 1 x TLA
Ligand TAR 1 x TAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P61
Unit cell:
a: 128.89Å b: 128.89Å c: 118.08Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.24
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Noroviruses everywhere: has something changed?
Estes et al. (2006)
10 more

8 mentions without citation

Viral enzymes.
Mesters et al. (2006)
7 more