PDBe 1wmh

X-ray diffraction
1.5Å resolution

Crystal structure of a PB1 domain complex of Protein kinase c iota and Par6 alpha

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein kinase C iota type Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 10.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P41743 (Residues: 25-108; Coverage: 14%)
Gene names: DXS1179E, PRKCI
Sequence domains: PB1 domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Partitioning defective 6 homolog alpha Chain: B
Molecule details ›
Chain: B
Length: 86 amino acids
Theoretical weight: 9.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q9NPB6 (Residues: 14-95; Coverage: 24%)
Gene names: PAR6A, PARD6A
Sequence domains: PB1 domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 94.15Å b: 38.363Å c: 45.478Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.224
Expression system: Escherichia coli BL21(DE3)