PDBe 1v7m

X-ray diffraction
2.51Å resolution

Human Thrombopoietin Functional Domain Complexed To Neutralizing Antibody TN1 Fab

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombopoietin Chains: V, X
Molecule details ›
Chains: V, X
Length: 163 amino acids
Theoretical weight: 17.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40225 (Residues: 22-184; Coverage: 49%)
Gene names: MGDF, THPO
Sequence domains: Erythropoietin/thrombopoietin
Structure domains: Growth Hormone; Chain: A;
Monoclonal TN1 Fab Heavy Chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 217 amino acids
Theoretical weight: 23.36 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Monoclonal TN1 Fab Light Chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 213 amino acids
Theoretical weight: 23.36 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 132.099Å b: 46.771Å c: 185.151Å
α: 90° β: 90.48° γ: 90°
R-values:
R R work R free
0.232 0.228 0.316
Expression systems:
  • Escherichia coli
  • Mus musculus