› 1uys

X-ray diffraction
2.8Å resolution

Acetyl-CoA carboxylase carboxyltransferase domain in complex with inhibitor haloxyfop

Released:
DOI: 10.2210/pdb1uys/pdb
PDB entry 1uys coloured by chain, front view.
PDB entry 1uys coloured by chain, side view.
PDB entry 1uys coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Molecular basis for the inhibition of the carboxyltransferase domain of acetyl-coenzyme-A carboxylase by haloxyfop and diclofop.
Zhang H, Tweel B, Tong L
Proc Natl Acad Sci U S A 101 5910-5 (2004)
PMID: 15079078

Function and Biology Details

Reactions catalysed: 
ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N(6)-L-lysine
ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169488 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA carboxylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 737 amino acids
Theoretical weight: 84.24 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00955 (Residues: 1482-2218; Coverage: 33%)
Gene names: ABP2, ACC1, FAS3, MTR7, N3175, YNR016C
Sequence domains: Carboxyl transferase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand H1L 3 x H1L
1 modified residue:
Ligand MSE 51 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 246.21Å b: 125.58Å c: 146.88Å
α: 90° β: 94.08° γ: 90°
R-values:
R R work R free
0.218 0.218 0.252
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Evolution in action: plants resistant to herbicides.
Powles et al. (2010)
16 more

6 mentions without citation

Molecular basis for the inhibition of the carboxyltransferase domain of acetyl-coenzyme-A carboxylase by haloxyfop and diclofop.
Zhang et al. (2004)
5 more