PDBe 1uef

X-ray diffraction
2.5Å resolution

Crystal Structure of Dok1 PTB Domain Complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Docking protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 14.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P97465 (Residues: 152-266; Coverage: 24%)
Gene names: Dok, Dok1
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Proto-oncogene tyrosine-protein kinase receptor Ret Chains: C, D
Molecule details ›
Chains: C, D
Length: 13 amino acids
Theoretical weight: 1.62 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P35546 (Residues: 1055-1067; Coverage: 1%)
Gene name: Ret

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 45.482Å b: 55.72Å c: 99.126Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.213 0.277
Expression systems:
  • Escherichia coli BL21
  • Not provided