› 1udc

X-ray diffraction
1.65Å resolution

STRUCTURE OF UDP-GALACTOSE-4-EPIMERASE COMPLEXED WITH UDP-MANNOSE

Released:
DOI: 10.2210/pdb1udc/pdb
PDB entry 1udc coloured by chain, front view.
PDB entry 1udc coloured by chain, side view.
PDB entry 1udc coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coli.
Thoden JB, Hegeman AD, Wesenberg G, Chapeau MC, Frey PA, Holden HM
Biochemistry 36 6294-304 (1997)
PMID: 9174344

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 37.31 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P09147 (Residues: 1-338; Coverage: 100%)
Gene names: JW0742, b0759, galD, galE
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
4 bound ligands:
Ligand NA 3 x NA
Ligand UFM 1 x UFM
Ligand PGE 1 x PGE
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 83.5Å b: 83.5Å c: 108.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.177 not available

Quick links

Citations

4 review citations

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
3 more

4 mentions without citation

BALBES: a molecular-replacement pipeline.
Long et al. (2008)
3 more