PDBe 1u0b

X-ray diffraction
2.3Å resolution

Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys

Released:
Primary publication:
Shape-selective RNA recognition by cysteinyl-tRNA synthetase.
Nat. Struct. Mol. Biol. 11 1134-41 (2004)
PMID: 15489861

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Cysteine--tRNA ligase Chain: B
Molecule details ›
Chain: B
Length: 461 amino acids
Theoretical weight: 52.27 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P21888 (Residues: 1-461; Coverage: 100%)
Gene names: JW0515, b0526, cysS
Sequence domains:
Structure domains:
Cysteinyl-tRNA synthetase Chain: A
Molecule details ›
Chain: A
Length: 74 nucleotides
Theoretical weight: 23.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P41212
Unit cell:
a: 129.49Å b: 129.49Å c: 91.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.29 0.233 0.277
Expression systems:
  • Not provided
  • Escherichia coli