› 1trn

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF HUMAN TRYPSIN 1: UNEXPECTED PHOSPHORYLATION OF TYROSINE 151

Released:
DOI: 10.2210/pdb1trn/pdb
PDB entry 1trn coloured by chain, front view.
PDB entry 1trn coloured by chain, side view.
PDB entry 1trn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human trypsin 1: unexpected phosphorylation of Tyr151.
Gaboriaud C, Serre L, Guy-Crotte O, Forest E, Fontecilla-Camps JC
J Mol Biol 259 995-1010 (1996)
PMID: 8683601

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 24.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P07477 (Residues: 24-247; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand ISP 2 x ISP
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P4
Unit cell:
a: 107.12Å b: 107.12Å c: 39.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 not available
Expression system: Not provided

Quick links

Citations

17 review citations

Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains.
Rabilloud T. (2002)
16 more

24 mentions without citation

Biochemical and structural insights into mesotrypsin: an unusual human trypsin.
Salameh et al. (2013)
23 more