PDBe 1tq7

X-ray diffraction
2.4Å resolution

Crystal structure of the anticoagulant thrombin mutant W215A/E217A bound to PPACK

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thrombin light chain Chain: A
Molecule details ›
Chain: A
Length: 44 amino acids
Theoretical weight: 5.15 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 320-363; Coverage: 7%)
Gene name: F2
Structure domains: Thrombin light chain domain
Thrombin heavy chain Chain: B
Molecule details ›
Chain: B
Length: 257 amino acids
Theoretical weight: 29.42 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 364-620; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 51.11Å b: 73.83Å c: 87.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.243
Expression system: Cricetulus griseus