PDBe 1tq0

X-ray diffraction
2.8Å resolution

Crystal structure of the potent anticoagulant thrombin mutant W215A/E217A in free form

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thrombin light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 31 amino acids
Theoretical weight: 3.65 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 333-363; Coverage: 5%)
Gene name: F2
Thrombin heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 257 amino acids
Theoretical weight: 29.42 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 364-620; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P213
Unit cell:
a: 131.93Å b: 131.93Å c: 131.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.292
Expression system: Cricetulus griseus