PDBe 1tmu

X-ray diffraction
2.5Å resolution

Changes in interactions in complexes of hirudin derivatives and human alpha-thrombin due to different crystal forms

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain Chain: L
Molecule details ›
Chain: L
Length: 28 amino acids
Theoretical weight: 3.32 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 333-360; Coverage: 5%)
Gene name: F2
Thrombin heavy chain Chain: H
Molecule details ›
Chain: H
Length: 259 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hirudin variant-2 Chain: J
Molecule details ›
Chain: J
Length: 11 amino acids
Theoretical weight: 1.49 KDa
Source organism: Hirudo medicinalis
Expression system: Not provided
UniProt:
  • Canonical: P09945 (Residues: 62-72; Coverage: 17%)

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 80.9Å b: 107.5Å c: 45.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 not available not available
Expression system: Not provided