PDBe 1t9g

X-ray diffraction
2.9Å resolution

Structure of the human MCAD:ETF complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 396 amino acids
Theoretical weight: 43.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11310 (Residues: 26-421; Coverage: 94%)
Gene name: ACADM
Sequence domains:
Structure domains:
Electron transfer flavoprotein subunit alpha, mitochondrial Chain: R
Molecule details ›
Chain: R
Length: 333 amino acids
Theoretical weight: 35.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13804 (Residues: 1-333; Coverage: 100%)
Gene name: ETFA
Sequence domains:
Structure domains: HUPs
Electron transfer flavoprotein subunit beta Chain: S
Molecule details ›
Chain: S
Length: 255 amino acids
Theoretical weight: 27.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P38117 (Residues: 1-255; Coverage: 100%)
Gene names: ETFB, FP585
Sequence domains: Electron transfer flavoprotein domain
Structure domains: HUPs

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 94.333Å b: 101.322Å c: 244.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.191 0.263
Expression system: Escherichia coli