PDBe 1t0k

X-ray diffraction
3.24Å resolution

Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex

Released:
Primary publication:
Joint X-ray and NMR refinement of the yeast L30e-mRNA complex.
Structure 12 1165-76 (2004)
PMID: 15242593

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (4 distinct):
60S ribosomal protein L30 Chain: B
Molecule details ›
Chain: B
Length: 105 amino acids
Theoretical weight: 11.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P14120 (Residues: 1-105; Coverage: 100%)
Gene names: RPL30, RPL32, YGL030W
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
Maltose-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 41.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II
5'-R(*GP*AP*CP*CP*GP*GP*AP*GP*UP*GP*UP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4.17 KDa
5'-R(*G*GP*AP*CP*GP*CP*AP*GP*AP*GP*AP*UP*GP*GP*UP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 5.22 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P41212
Unit cell:
a: 136.006Å b: 136.006Å c: 123.813Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.261 0.32
Expression system: Escherichia coli