PDBe 1syq

X-ray diffraction
2.42Å resolution

Human vinculin head domain VH1, residues 1-258, in complex with human talin's vinculin binding site 1, residues 607-636

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis for amplifying vinculin activation by talin.
J. Biol. Chem. 279 27667-78 (2004)
PMID: 15070891

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero 12-mer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Talin-1 Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 2.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9Y490 (Residues: 607-631; Coverage: 1%)
Gene names: KIAA1027, TLN, TLN1
Vinculin Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 29.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P18206 (Residues: 1-258; Coverage: 23%)
Gene name: VCL
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R32
Unit cell:
a: 141.098Å b: 141.098Å c: 104.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.188 0.236
Expression systems:
  • Not provided
  • Escherichia coli BL21