PDBe 1suw

X-ray diffraction
2.45Å resolution

Crystal structure of a NAD kinase from Archaeoglobus fulgidus in complex with its substrate and product: Insights into the catalysis of NAD kinase

Released:

Function and Biology Details

Reaction catalysed:
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 249 amino acids
Theoretical weight: 27.91 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30297 (Residues: 1-249; Coverage: 100%)
Gene names: AF_2373, nadK
Sequence domains: ATP-NAD kinase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P41212
Unit cell:
a: 122.141Å b: 122.141Å c: 198.595Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.252
Expression system: Escherichia coli