PDBe 1sk6

X-ray diffraction
3.2Å resolution

Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin, 3',5' cyclic AMP (cAMP), and pyrophosphate


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-sensitive adenylate cyclase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 510 amino acids
Theoretical weight: 58.81 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
  • Canonical: P40136 (Residues: 291-800; Coverage: 67%)
Gene names: BXA0141, GBAA_pXO1_0142, cya, pXO1-122
Sequence domains: Anthrax toxin LF subunit
Structure domains:
Calmodulin-2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Homo sapiens
  • Canonical: P0DP24 (Residues: 2-149; Coverage: 99%)
Gene names: CALM2, CAM2, CAMB
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 116.87Å b: 166.453Å c: 342.393Å
α: 90° β: 90° γ: 90°
R R work R free
0.251 0.25 0.307
Expression system: Escherichia coli