PDBe 1s78

X-ray diffraction
3.25Å resolution

Insights into ErbB signaling from the structure of the ErbB2-pertuzumab complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Pertuzumab Fab heavy chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 226 amino acids
Theoretical weight: 24.16 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Pertuzumab Fab light chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 214 amino acids
Theoretical weight: 23.55 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z3Y4 (Residues: 23-236; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
Receptor tyrosine-protein kinase erbB-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 624 amino acids
Theoretical weight: 68.79 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04626 (Residues: 23-646; Coverage: 51%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P43212
Unit cell:
a: 139.412Å b: 139.412Å c: 356.873Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.268
Expression systems:
  • Escherichia coli
  • Cricetulus griseus