› 1s3g

X-ray diffraction
2.25Å resolution

Crystal structure of adenylate kinase from Bacillus globisporus

Released:
DOI: 10.2210/pdb1s3g/pdb
PDB entry 1s3g coloured by chain, front view.
PDB entry 1s3g coloured by chain, side view.
PDB entry 1s3g coloured by chain, top view.
Source organism: Sporosarcina globispora
Primary publication:
Structures and analysis of highly homologous psychrophilic, mesophilic, and thermophilic adenylate kinases.
Bae E, Phillips GN
J Biol Chem 279 28202-8 (2004)
PMID: 15100224

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160838 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 23.92 KDa
Source organism: Sporosarcina globispora
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84139 (Residues: 1-217; Coverage: 100%)
Gene name: adk
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand AP5 1 x AP5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P3121
Unit cell:
a: 65.288Å b: 65.288Å c: 94.378Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Cold-adapted enzymes.
Siddiqui et al. (2006)
9 more

11 mentions without citation

Psychrophilic enzymes: from folding to function and biotechnology.
Feller G. (2013)
10 more