PDBe 1s1q

X-ray diffraction
2Å resolution

TSG101(UEV) domain in complex with Ubiquitin

Released:
Source organism: Homo sapiens
Primary publication:
Ubiquitin recognition by the human TSG101 protein.
Mol. Cell 13 783-9 (2004)
PMID: 15053872

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor susceptibility gene 101 protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 145 amino acids
Theoretical weight: 16.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99816 (Residues: 1-145; Coverage: 37%)
Gene name: TSG101
Sequence domains: UEV domain
Structure domains: Ubiquitin Conjugating Enzyme
Ubiquitin Chains: B, D
Molecule details ›
Chains: B, D
Length: 76 amino acids
Theoretical weight: 8.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 143.634Å b: 59.154Å c: 93.998Å
α: 90° β: 128.67° γ: 90°
R-values:
R R work R free
0.191 0.201 0.24
Expression system: Escherichia coli