› 1s1m

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141591 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 545 amino acids
Theoretical weight: 60.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7E5 (Residues: 1-545; Coverage: 100%)
Gene names: JW2751, b2780, pyrG
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 4 x SO4
Ligand MG 4 x MG
Ligand IOD 16 x IOD
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1, null
Spacegroup: P21212
Unit cell:
a: 165.507Å b: 106.807Å c: 130.033Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.281
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Asparagine synthetase chemotherapy.
Richards et al. (2006)
10 more

11 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
10 more