PDBe 1ryf

X-ray diffraction
1.75Å resolution

Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase

Released:

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related C3 botulinum toxin substrate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 22.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 1-75, 76-182; Coverage: 95%)
  • Best match: P63000-2 (Residues: 1-201)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 51.64Å b: 78.69Å c: 96.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.223
Expression system: Escherichia coli