› 1rp5

X-ray diffraction
3Å resolution

PBP2x from Streptococcus pneumoniae strain 5259 with reduced susceptibility to beta-lactam antibiotics

Released:
DOI: 10.2210/pdb1rp5/pdb
PDB entry 1rp5 coloured by chain, front view.
PDB entry 1rp5 coloured by chain, side view.
PDB entry 1rp5 coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
A PBP2x from a clinical isolate of Streptococcus pneumoniae exhibits an alternative mechanism for reduction of susceptibility to beta-lactam antibiotics.
Pernot L, Chesnel L, Le Gouellec A, Croizé J, Vernet T, Dideberg O, Dessen A
J Biol Chem 279 16463-70 (2004)
PMID: 14734544

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein 2x Chains: A, B
Molecule details ›
Chains: A, B
Length: 702 amino acids
Theoretical weight: 76.91 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P14677 (Residues: 49-750; Coverage: 94%)
Gene names: SP_0336, pbpX
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P4222
Unit cell:
a: 194.989Å b: 194.989Å c: 154.245Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.231 0.256
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Penicillin-binding proteins and beta-lactam resistance.
Zapun et al. (2008)
8 more

4 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
3 more