PDBe 1r3j

X-ray diffraction
1.9Å resolution

potassium channel KcsA-Fab complex in high concentration of Tl+

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa constant Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-105; Coverage: 98%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig gamma-1 chain C region secreted form Chain: B
Molecule details ›
Chain: B
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01868 (Residues: 1-101; Coverage: 31%)
Gene names: Igh-4, Ighg1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.21 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 1-124; Coverage: 78%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: I4
Unit cell:
a: 154.74Å b: 154.74Å c: 76.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.228
Expression system: Escherichia coli