PDBe 1qrs

X-ray diffraction
2.6Å resolution

GLUTAMINYL-TRNA SYNTHETASE MUTANT D235N COMPLEXED WITH GLUTAMINE TRANSFER RNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Glutamine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 553 amino acids
Theoretical weight: 63.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00962 (Residues: 2-554; Coverage: 100%)
Gene names: JW0666, b0680, glnS
Sequence domains:
Structure domains:
TRNAGLN2 Chain: B
Molecule details ›
Chain: B
Length: 75 nucleotides
Theoretical weight: 24.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 243.7Å b: 93.7Å c: 115.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 not available
Expression system: Escherichia coli