PDBe 1qps

X-ray diffraction
2.5Å resolution

THE CRYSTAL STRUCTURE OF A POST-REACTIVE COGNATE DNA-ECO RI COMPLEX AT 2.50 A IN THE PRESENCE OF MN2+ ION

Released:
Source organism: Escherichia coli
Entry authors: Horvath M, Choi J, Kim Y, Wilkosz P, Rosenberg JM

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Type-2 restriction enzyme EcoRI Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 29.17 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P00642 (Residues: 17-277; Coverage: 94%)
Gene name: ecoRIR
Sequence domains: Restriction endonuclease EcoRI
Structure domains: Eco RI Endonuclease, subunit A
5'-D(*TP*CP*GP*CP*GP*)-3' Chain: M
Molecule details ›
Chain: M
Length: 5 nucleotides
Theoretical weight: 1.5 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*AP*AP*TP*TP*CP*GP*CP*GP*)-3' Chain: N
Molecule details ›
Chain: N
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P321
Unit cell:
a: 118.4Å b: 118.4Å c: 49.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 not available not available
Expression system: Not provided