› 1qjb

X-ray diffraction
2Å resolution

14-3-3 ZETA/PHOSPHOPEPTIDE COMPLEX (MODE 1)

Released:
DOI: 10.2210/pdb1qjb/pdb
PDB entry 1qjb coloured by chain, front view.
PDB entry 1qjb coloured by chain, side view.
PDB entry 1qjb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of 14-3-3 phosphopeptide complexes identifies a dual role for the nuclear export signal of 14-3-3 in ligand binding.
Rittinger K, Budman J, Xu J, Volinia S, Cantley LC, Smerdon SJ, Gamblin SJ, Yaffe MB
Mol Cell 4 153-66 (1999)
PMID: 10488331

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158972 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: P63104 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
PHOSPHOPEPTIDE Chains: Q, S
Molecule details ›
Chains: Q, S
Length: 8 amino acids
Theoretical weight: 970 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P212121
Unit cell:
a: 68.35Å b: 71.98Å c: 131Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.288
Expression systems:
  • Escherichia coli DH5[alpha]
  • Not provided

Quick links

Citations

57 review citations

14-3-3 proteins: structure, function, and regulation.
Fu et al. (2000)
56 more

25 mentions without citation

Structural basis for regulation of RNA-binding proteins by phosphorylation.
Thapar R. (2015)
24 more