PDBe 1qge

X-ray diffraction
1.7Å resolution

NEW CRYSTAL FORM OF PSEUDOMONAS GLUMAE (FORMERLY CHROMOBACTERIUM VISCOSUM ATCC 6918) LIPASE

Released:
Source organism: Burkholderia glumae
Entry authors: Lang DA, Stadler P, Kovacs A, Paltauf F, Dijkstra BW

Function and Biology Details

Reaction catalysed:
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Triacylglycerol lipase Chain: D
Molecule details ›
Chain: D
Length: 222 amino acids
Theoretical weight: 22.95 KDa
Source organism: Burkholderia glumae
UniProt:
  • Canonical: Q05489 (Residues: 40-261; Coverage: 70%)
Gene names: lip, lipA
Structure domains: Rossmann fold
Triacylglycerol lipase Chain: E
Molecule details ›
Chain: E
Length: 97 amino acids
Theoretical weight: 10.18 KDa
Source organism: Burkholderia glumae
UniProt:
  • Canonical: Q05489 (Residues: 262-358; Coverage: 30%)
Gene names: lip, lipA

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 40.98Å b: 43.35Å c: 140.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.187 0.228