PDBe 1qew

X-ray diffraction
2.2Å resolution

HUMAN CLASS I HISTOCOMPATIBILITY ANTIGEN (HLA-A 0201) COMPLEX WITH A NONAMERIC PEPTIDE FROM MELANOMA-ASSOCIATED ANTIGEN 3 (RESIDUES 271-279)

Released:
Source organism: Homo sapiens
Entry authors: Orth P, Alings C, Saenger W, Ziegler A

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Melanoma-associated antigen 3 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.06 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P43357 (Residues: 271-279; Coverage: 3%)
Gene names: MAGE3, MAGEA3

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-21
Spacegroup: P21
Unit cell:
a: 58.99Å b: 54.52Å c: 76.38Å
α: 90° β: 105.67° γ: 90°
R-values:
R R work R free
0.204 0.204 0.242
Expression system: Escherichia coli