PDBe 1q9x

X-ray diffraction
2.69Å resolution

Crystal structure of Enterobacteria phage RB69 gp43 DNA polymerase complexed with tetrahydrofuran containing DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 903 amino acids
Theoretical weight: 104.66 KDa
Source organism: Enterobacteria phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38087 (Residues: 1-903; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:
5'-GCGGACTGCTTAC(dideoxycytidine)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 18 nucleotides
Theoretical weight: 5.57 KDa
Source organism: Enterobacteria phage RB69
Expression system: Not provided
5'-AC(tetrahydrofuran)GGTAAGCAGTCCGCGG-3' Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Enterobacteria phage RB69
Expression system: Not provided

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21
Unit cell:
a: 131.948Å b: 122.237Å c: 165.358Å
α: 90° β: 96.85° γ: 90°
R-values:
R R work R free
0.219 0.215 0.288
Expression systems:
  • Escherichia coli
  • Not provided