PDBe 1q90

X-ray diffraction
3.1Å resolution

Structure of the cytochrome b6f (plastohydroquinone : plastocyanin oxidoreductase) from Chlamydomonas reinhardtii

Released:
Source organism: Chlamydomonas reinhardtii
Primary publication:
An atypical haem in the cytochrome b(6)f complex.
Nature 426 413-8 (2003)
PMID: 14647374

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero 18-mer (preferred)
hetero 36-mer
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Cytochrome f Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 32.11 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P23577 (Residues: 32-317; Coverage: 100%)
Gene name: petA
Sequence domains:
Structure domains:
Cytochrome b6 Chain: B
Molecule details ›
Chain: B
Length: 215 amino acids
Theoretical weight: 24.18 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q00471 (Residues: 1-215; Coverage: 100%)
Gene name: petB
Sequence domains: Cytochrome b/b6/petB
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic Chain: C
Molecule details ›
Chain: C
Length: 127 amino acids
Theoretical weight: 13.66 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P49728 (Residues: 80-206; Coverage: 62%)
Gene name: petC
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain
Cytochrome b6-f complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 159 amino acids
Theoretical weight: 17.31 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P23230 (Residues: 1-159; Coverage: 99%)
Gene name: petD
Sequence domains: Cytochrome b(C-terminal)/b6/petD
Structure domains:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic Chain: R
Molecule details ›
Chain: R
Length: 49 amino acids
Theoretical weight: 4.71 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P49728 (Residues: 31-79; Coverage: 24%)
Gene name: petC
Sequence domains: Cytochrome B6-F complex Fe-S subunit
Cytochrome b6-f complex subunit 5 Chain: G
Molecule details ›
Chain: G
Length: 37 amino acids
Theoretical weight: 3.98 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q08362 (Residues: 1-37; Coverage: 100%)
Gene name: petG
Sequence domains: Cytochrome B6-F complex subunit 5
Cytochrome b6-f complex subunit 6 Chain: L
Molecule details ›
Chain: L
Length: 32 amino acids
Theoretical weight: 3.44 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P50369 (Residues: 12-43; Coverage: 74%)
Gene name: petL
Sequence domains: Cytochrome B6-F complex subunit VI (PetL)
Cytochrome b6-f complex subunit 7, chloroplastic Chain: M
Molecule details ›
Chain: M
Length: 39 amino acids
Theoretical weight: 4.04 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q42496 (Residues: 61-99; Coverage: 39%)
Gene names: petM, petX
Sequence domains: PetM family of cytochrome b6f complex subunit 7
Cytochrome b6-f complex subunit 8, chloroplastic Chain: N
Molecule details ›
Chain: N
Length: 31 amino acids
Theoretical weight: 3.28 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P0C1D4 (Residues: 5-35; Coverage: 89%)
Gene names: CHLREDRAFT_177155, PETN
Sequence domains: PetN

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: I222
Unit cell:
a: 102.454Å b: 171.205Å c: 351.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.261
Expression system: Chlamydomonas reinhardtii