PDBe 1q5w

Solution NMR

Ubiquitin Recognition by Npl4 Zinc-Fingers

Released:
Source organisms:
Primary publication:
Ubiquitin interactions of NZF zinc fingers.
EMBO J. 23 1411-21 (2004)
PMID: 15029239

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear protein localization protein 4 homolog Chain: A
Molecule details ›
Chain: A
Length: 31 amino acids
Theoretical weight: 3.38 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ES54 (Residues: 580-608; Coverage: 5%)
Gene names: Npl4, Nploc4
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Torsion angle dynamics followed by simulated annealing
Expression system: Escherichia coli BL21(DE3)