› 1q1r

X-ray diffraction
1.91Å resolution

Crystal Structure of Putidaredoxin Reductase from Pseudomonas putida

Released:
DOI: 10.2210/pdb1q1r/pdb
PDB entry 1q1r coloured by chain, front view.
PDB entry 1q1r coloured by chain, side view.
PDB entry 1q1r coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Crystal structure of putidaredoxin reductase from Pseudomonas putida, the final structural component of the cytochrome P450cam monooxygenase.
Sevrioukova IF, Li H, Poulos TL
J Mol Biol 336 889-902 (2004)
PMID: 15095867

Function and Biology Details

Reaction catalysed: 
Reduced putidaredoxin + NAD(+) = oxidized putidaredoxin + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147783 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putidaredoxin reductase CamA Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 46.78 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16640 (Residues: 1-422; Coverage: 100%)
Gene name: camA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 70.78Å b: 104.13Å c: 166.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.236
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Variations on a (t)heme--novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamily.
Munro et al. (2007)
5 more

9 mentions without citation

Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation.
Begara-Morales et al. (2015)
8 more