› 1poc

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF BEE-VENOM PHOSPHOLIPASE A2 IN A COMPLEX WITH A TRANSITION-STATE ANALOGUE

Released:
DOI: 10.2210/pdb1poc/pdb
PDB entry 1poc coloured by chain, front view.
PDB entry 1poc coloured by chain, side view.
PDB entry 1poc coloured by chain, top view.
Source organism: Apis mellifera
Primary publication:
Crystal structure of bee-venom phospholipase A2 in a complex with a transition-state analogue.
Scott DL, Otwinowski Z, Gelb MH, Sigler PB
Science 250 1563-6 (1990)
PMID: 2274788

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132761 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2 Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 15.27 KDa
Source organism: Apis mellifera
Expression system: Not provided
UniProt:
  • Canonical: P00630 (Residues: 34-167; Coverage: 90%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GEL 1 x GEL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I4122
Unit cell:
a: 89.5Å b: 89.5Å c: 132.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 not available
Expression system: Not provided

Quick links

Citations

17 review citations

Increasing molecular diversity of secreted phospholipases A(2) and their receptors and binding proteins.
Valentin et al. (2000)
16 more

38 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
37 more