PDBe 1p27

X-ray diffraction
2Å resolution

Crystal Structure of the Human Y14/Magoh complex

Released:
Source organism: Homo sapiens
Primary publication:
Structure of the Y14-Magoh core of the exon junction complex.
Curr. Biol. 13 933-41 (2003)
PMID: 12781131

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein mago nashi homolog Chains: A, C
Molecule details ›
Chains: A, C
Length: 144 amino acids
Theoretical weight: 16.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61326 (Residues: 2-145; Coverage: 99%)
Gene names: MAGOH, MAGOHA
Structure domains: Mago nashi
RNA-binding protein 8A Chains: B, D
Molecule details ›
Chains: B, D
Length: 106 amino acids
Theoretical weight: 12.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5S9 (Residues: 50-155; Coverage: 61%)
Gene names: HSPC114, MDS014, RBM8, RBM8A
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 47.15Å b: 108.455Å c: 50.942Å
α: 90° β: 90.24° γ: 90°
R-values:
R R work R free
0.222 0.22 0.268
Expression system: Escherichia coli BL21(DE3)