PDBe 1ozj

X-ray diffraction
2.4Å resolution

Crystal structure of Smad3-MH1 bound to DNA at 2.4 A resolution

Released:
Source organism: Homo sapiens

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mothers against decapentaplegic homolog 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P84022 (Residues: 1-144; Coverage: 34%)
Gene names: MADH3, SMAD3
Sequence domains: MH1 domain
Structure domains: SMAD MH1 domain
Smad binding element Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
Smad binding element Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.62 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 45.6Å b: 60.4Å c: 71.6Å
α: 90° β: 102° γ: 90°
R-values:
R R work R free
0.22 0.21 0.27
Expression systems:
  • Escherichia coli
  • Not provided