PDBe 1ow6

X-ray diffraction
2.35Å resolution

Paxillin LD4 motif bound to the Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Paxillin Chains: D, F
Molecule details ›
Chains: D, F
Length: 13 amino acids
Theoretical weight: 1.44 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P49023 (Residues: 262-274; Coverage: 2%)
Gene name: PXN
Focal adhesion kinase 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 161 amino acids
Theoretical weight: 17.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05397 (Residues: 892-1052; Coverage: 15%)
Gene names: FAK, FAK1, PTK2
Sequence domains: Focal adhesion targeting region
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 88.308Å b: 223.274Å c: 96.992Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.242 0.274
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)