› 1orc

X-ray diffraction
1.54Å resolution

CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]

Released:
DOI: 10.2210/pdb1orc/pdb
PDB entry 1orc coloured by chain, front view.
PDB entry 1orc coloured by chain, side view.
PDB entry 1orc coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
High-resolution structure of an engineered Cro monomer shows changes in conformation relative to the native dimer.
Albright RA, Mossing MC, Matthews BW
Biochemistry 35 735-42 (1996)
PMID: 8547253

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein cro Chain: A
Molecule details ›
Chain: A
Length: 71 amino acids
Theoretical weight: 7.91 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03040 (Residues: 1-53, 54-66; Coverage: 100%)
Gene names: cro, lambdap57
Sequence domains: Cro
Structure domains: CRO Repressor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 34.77Å b: 39.17Å c: 48.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 not available
Expression system: Escherichia coli

Quick links

Citations

5 review citations

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
4 more

17 mentions without citation

Interplay of self-association and conformational flexibility in regulating protein function.
Garton et al. (2018)
16 more