PDBe 1ook

X-ray diffraction
2.3Å resolution

Crystal Structure of the Complex of Platelet Receptor GPIb-alpha and Human alpha-Thrombin

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Thrombin light chain Chain: A
Molecule details ›
Chain: A
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Thrombin heavy chain Chain: B
Molecule details ›
Chain: B
Length: 259 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
PHE-PRO-ARG-Chloromethylketone Chain: P
Molecule details ›
Chain: P
Length: 3 amino acids
Theoretical weight: 419 Da
Glycocalicin Chain: G
Molecule details ›
Chain: G
Length: 290 amino acids
Theoretical weight: 32.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07359 (Residues: 17-306; Coverage: 46%)
Gene name: GP1BA
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P43212
Unit cell:
a: 67.649Å b: 67.649Å c: 328.578Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.26
Expression system: Escherichia coli