PDBe 1obc

X-ray diffraction
2.1Å resolution

LEUCYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH A POST-TRANSFER EDITING SUBSTRATE ANALOGUE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 878 amino acids
Theoretical weight: 101.17 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72GM3 (Residues: 1-878; Coverage: 100%)
Gene names: TT_C1825, leuS
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 101.896Å b: 154.81Å c: 175.118Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.225
Expression system: Escherichia coli BL21(DE3)