PDBe 1oaf

X-ray diffraction
1.4Å resolution

Ascobate peroxidase from soybean cytosol in complex with ascorbate

Released:
Source organism: Glycine max
Primary publication:
Crystal structure of the ascorbate peroxidase-ascorbate complex.
Nat. Struct. Biol. 10 303-7 (2003)
PMID: 12640445

Function and Biology Details

Reaction catalysed:
(1a) 2 L-ascorbate + H(2)O(2) + 2 H(+) = 2 monodehydroascorbate + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic ascorbate peroxidase 1 Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.36 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: Q43758 (Residues: 2-250; Coverage: 100%)
Gene names: 553156, apx1
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM

Cofactor: Ligand ASC 1 x ASC
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P42212
Unit cell:
a: 81.328Å b: 81.328Å c: 74.884Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.196
Expression system: Escherichia coli