PDBe 1o6o

X-ray diffraction
2.8Å resolution

Importin Beta aa1-442 bound to five FxFG repeats from yeast Nsp1p. Second crystal form

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit beta-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 442 amino acids
Theoretical weight: 49.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14974 (Residues: 1-442; Coverage: 51%)
  • Best match: Q14974-2 (Residues: 1-297)
Gene names: KPNB1, NTF97
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Nucleoporin NSP1 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 119 amino acids
Theoretical weight: 12.55 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14907 (Residues: 496-608; Coverage: 14%)
Gene names: J1207, NSP1, YJL041W

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 67.11Å b: 125.35Å c: 266.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.273
Expression system: Escherichia coli BL21(DE3)