PDBe 1nvw

X-ray diffraction
2.7Å resolution

Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero 24-mer
hetero 12-mer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase HRas, N-terminally processed Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Son of sevenless homolog 1 Chain: S
Molecule details ›
Chain: S
Length: 481 amino acids
Theoretical weight: 56.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07889 (Residues: 566-1046; Coverage: 36%)
Gene name: SOS1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: I422
Unit cell:
a: 184.046Å b: 184.046Å c: 178.943Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.246
Expression system: Escherichia coli BL21(DE3)