PDBe 1nvm

X-ray diffraction
1.7Å resolution

Crystal structure of a bifunctional aldolase-dehydrogenase : sequestering a reactive and volatile intermediate

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
4-hydroxy-2-oxovalerate aldolase Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 345 amino acids
Theoretical weight: 37.52 KDa
Source organism: Pseudomonas sp. CF600
Expression system: Escherichia coli
UniProt:
  • Canonical: P51016 (Residues: 1-345; Coverage: 100%)
Gene name: dmpG
Sequence domains:
Structure domains:
Acetaldehyde dehydrogenase Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 312 amino acids
Theoretical weight: 32.71 KDa
Source organism: Pseudomonas sp. CF600
Expression system: Escherichia coli
UniProt:
  • Canonical: Q52060 (Residues: 1-312; Coverage: 100%)
Gene name: dmpF
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 3 x NAD

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 102.2Å b: 140Å c: 191.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.23
Expression system: Escherichia coli