PDBe 1nui

X-ray diffraction
2.9Å resolution

Crystal Structure of the primase fragment of Bacteriophage T7 primase-helicase protein

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase/helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 28.4 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03692 (Residues: 1-255; Coverage: 45%)
Gene name: 4
Sequence domains: Zinc-binding domain of primase-helicase
Structure domains: N-terminal domain of TfIIb

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P3121
Unit cell:
a: 136.459Å b: 136.46Å c: 85.445Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.235 0.277
Expression system: Escherichia coli BL21(DE3)