PDBe 1nu9

X-ray diffraction
2.2Å resolution

Staphylocoagulase-Prethrombin-2 complex

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thrombin heavy chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 291 amino acids
Theoretical weight: 33.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 332-622; Coverage: 49%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Staphylocoagulase Chains: C, F
Molecule details ›
Chains: C, F
Length: 281 amino acids
Theoretical weight: 33.21 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q846V4 (Residues: 1-281; Coverage: 43%)
Sequence domains: Staphylococcus aureus coagulase
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2
Unit cell:
a: 181.16Å b: 102.34Å c: 135.54Å
α: 90° β: 129.91° γ: 90°
R-values:
R R work R free
0.217 0.21 0.253
Expression system: Escherichia coli BL21