PDBe 1nu7

X-ray diffraction
2.2Å resolution

Staphylocoagulase-Thrombin Complex

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 28 amino acids
Theoretical weight: 3.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 332-359; Coverage: 5%)
Gene name: F2
Thrombin heavy chain Chains: B, F
Molecule details ›
Chains: B, F
Length: 259 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Staphylocoagulase Chains: D, H
Molecule details ›
Chains: D, H
Length: 282 amino acids
Theoretical weight: 33.34 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q846V4 (Residues: 1-281; Coverage: 43%)
Sequence domains: Staphylococcus aureus coagulase
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2
Unit cell:
a: 180.17Å b: 102Å c: 135.3Å
α: 90° β: 130.08° γ: 90°
R-values:
R R work R free
0.209 0.209 0.249
Expression system: Escherichia coli BL21